The stage follows the same route as the Gran Fondo Nove Colli. With the exception of the first and of the last 25 km, the remaining 150 km do not even feature a single flat metre to let the bunch catch their breath. The route is an endless succession of climbs and descents, with double-digit gradients at points, often on narrow and worn-out roads.
From 5 to 3 km remaining to the finish, the road is relatively wide and mainly straight, with several roundabouts. With 2.5 km to go, the route bends as it descends from the railway flyover, and then passes underneath, onto a narrow roundabout. With 1.4 km to go, one last left-hand bend leads into the home straight (featuring two speed bumps), on 7.5 m wide tarmac.